![]() ![]() (1968) A critical survey of European taxa in Ulvales. Capsosiphon, Percursaria, Blidingia, Enteromorpha. Comparison with previous reports from other methods for the physical investigation of nuclear genomes shows that flow cytometry of nuclei isolated from protoplasts is an accurate, convenient and robust technique to assay for genome sizes and base pair composition in marine macroalgae.īliding C (1963) A critical survey of European taxa in Ulvales. ![]() The second model, based on the curvilinear relationships Hoechst 33342 fluorescence/A-T content and mithramycin A fluorescence/G-C content, resulted in comparatively more homogenous and consistent data and appears more accurate. The first one was based on the linear relationships mithramycin A fluorescence/G-C content and ethidium bromide fluorescence/total DNA content. ![]() ![]() Two models were used for the estimation of the proportion of guanine plus cytosine in the nuclear genome. GC% analysis was based on staining with either Hoechst 33342 or mithramycin A, two fluorochromes specific for the bases A-T and G-C, respectively. The size of the nuclear genome ranged from 0.13 pg per cell in the 1 C population of Ulva rigida to 3.40 pg per cell in the 2 C population of Sphacelaria sp. Genome size was determined from the fluorescence of the nuclei stained with ethidium bromide. The amounts of nuclear DNA in ten species of seaweeds belonging to the Rhodophyceae, Phaeophyceae, and Chlorophyceae were determined by flow cytometric analysis of nuclei isolated from protoplasts. ![]()
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